In situ Biomolecule Assembly in Completely Packaged Microfluidic Devices Xiaolong Luo

Fig. 1 Fluorescence image of assembly site (a) after glutaraldehyde reaction (b) after GFP reaction (c) negative control (no bias) (d) GFP conjugated chitosan film. (e) Image J fluorescence surface plot

Objective
To demonstrate in-situ biomolecule assembly in completely packaged microfluidic enviroment.

Methods
Electrodeposited chitosan forms the scaffold for biomolecule assembly at specific sites in μfluidics.

Results
Both in situ fluorescence and ex situ profilometer results confirm chitosan-mediated in situ biomolecule assembly; the biological activities of assembled molecules is preserved.

Future Work
To study in-situ enzymatic bio-reactions and real-time protein synthesis in completely packaged μfluidics.